Usage of SESA:

SESA is used to select the optimal antibody targeting specific epitopes from an antibody library. To address different application scenarios, we provide three input modes. just click the corresponding "quickly start" button.

  1. Querying the built-in structure-antibody library (Including 2867 unique antibody PDB structures).
  2. Querying the user submitted antibody structure library.
  3. Querying the user submitted antibody sequence library.




While using the build-in structure-antibody library mode, please provide the following information:




While submitting the user-defined structure-antibody library for querying, please provide the following information:
(You need to save the antibody CDR structures as separate '.pdb' files, then package them into a '.zip' file for upload.)




While submitting the user-defined structure-antibody library for querying, please provide the following information:
(You need to save the antibody CDR structures as separate '.pdb' files, then package them into a '.zip' file for upload.)




Once the information is successfully submitted, SESA will then redirect to the following page and perform calculations in the backend.




The result page will automatically redirect once the calculations are completed. This page includes an antibody ranking table, where antibodies are arranged in descending order based on the SESA score. If you are using our build-in structure-antibody library, this table will also include sequence information for each antibody and their corresponding PDBID.

You can also manually check the results by copying the job ID generated on the calculation page. The result files will also be sent to your email if you provided one.

Note:

1. For commercial use, please contact us via email: zwcao@fudan.edu.cn.

2. We welcome files smaller than 50 MB. For large-scale antibody screening and multi-epitope batch screening, please contact us via email: zwcao@fudan.edu.cn.

2. We have also generated many other antibody libraries, such as large-scale human-simulated antibody library (n=3.24 × 10⁸) and mouse-simulated antibody library (n=1.60 × 10⁷) (see our article). If you have any related antibody library screening needs, please also contact us via email: zwcao@fudan.edu.cn.