Antibody (Ab) plays critical roles in both therapeutic and diagnostic applications. Though antibody engineering has enabled high yield of antigen-specific Ab binders, further identification of functional ones, which are often relevant to conformational epitopes, remains a time and cost-intensive endeavor.

Here, we proposed an in-silico tool, SESA, to screen those Abs targeting a pre-defined epitope area by calculating the physio-chemical complementarity between epitopes and paratope pairs.